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The sections were then blocked in blocking solution (PBS, 0.1% Tween-20, 1% donkey serum, 5% FCS) for 1 h, followed by incubation with the primary antibodies overnight at 4 C
At the same time, single-cell epigenomic studies suggest that microglia and astrocytes can enter long-lasting, reprogrammed states, retaining aspects of prior immune experience rather than returning to a naive baseline
By regulating appetite, boosting metabolism, and stabilizing blood sugar, GLP-1s could play a key role in easing some challenges of menopause