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doi:10.1016/j.jconrel.2011.01.030

Further quality filtering, including removal of poly-N and low-quality reads, was conducted using NGSQCToolkit version 2.3.3 ( Cleaned reads were aligned to the human reference genome GRCh38.97 (Accession number: GCA_000001405.27) using HISAT2 version 2.1.0 ( Transcript abundance was calculated as fragments per kilobase of transcript per million mapped reads (FPKM) using StringTie version 2.2.1 ( which also provided normalized expression values

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