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For the synchronization experiments, cells were synchronized with dexamethasone (100 nM, 30 min) on day 6 of differentiation and assayed or collected at the indicated time points

This level of precision is overkill for standard dosing but invaluable for microdosing protocols where researchers work with doses of 0.25 mg to 1 mg

MOTS-c is often provided as a lyophilized powder that must be reconstituted with bacteriostatic water using sterile technique before use

Several GPs that showed significant fold changes in subjects with metabolic syndrome had also shown similar changes (e.g., increase or decrease) at the end of 4th week during 30-day intermittent fasting (KIT, CROCC, DNTT, POLK, SRGN, CLSTN1) and 1 week after 30-day intermittent fasting (PRKCSH, CALU, SPECC1L, IGFBP4, MYH7, CDH6, H2B histone, PKP1, LRRC3, FGB, ENPP2) in our previous study conducted in healthy subjects although they had not reached statistical significance 15