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Loss of SQLE expression did not affect host cell viability (Fig S3C), and Cryptosporidium growth and development was indeed significantly enhanced in SQLE -KO cells compared to WT controls (Fig 3A)

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The expression level of rHrGSTm1 in both the supernatant and precipitate was analyzed by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE)