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The extraction solvent consisted of 80% methanol and 20% aqueous solution (pH 7.00) containing 10 mM ammonium formate (Sigma-Aldrich, 70221-25G-F) and 25 mM NEM (Thermo Scientific, 040526.06)
The patients entered into the trial had to meet the following inclusion criteria: - erectile dysfunction secondary to radical retropubic prostatectomy, with or without bilateral sparing of the neurovascular bundles, in patients in whom the prostatectomy had been performed at least 6 months but less than 2 years prior to entry into the clinical trial
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