If you feel fatigued, numb, or foggy despite healthy habits, a B12 deficiency could be the cause
A simplified framework is that its thought to occur when the intensity, frequency, and volume of Achilles tendon loading, such as during walking, running, or playing sports, exceeds your capacity to recover and adapt appropriately
Excess media was removed and replaced with 70% L15 media containing 10 M DCFH-DA and incubated at 20C for 30 min (32), after which the solution was removed, replaced with 10 g/ml Calcofluor White (CFW) to stain Bd for 5 mins (28), rinsed and replaced with 70% L15 media
Allow both the vial and bacteriostatic water to reach room temperature