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Ethical principles of the study complied with the European Directive 010/63/E, the Law on Amendments to the Animal Protection Act (Official Gazette 37/13), the Animal Protection Act (Official Gazette 135/06), the ordinance on the protection of animals used for scientific purposes (Official Gazette 55/13), Federation of European Laboratory Animal Science Associations (FELASA) recommendations, and the recommendations of the Ethics Committee of the School of Medicine, University of Zagreb

Step 3: Monitor for 4 Hours Given the sub-60-minute half-life, most pharmacologic activity will have ceased within 3 to 4 hours

Bioavailability of GHK-Cu Bioavailability is how much of a compound actually reaches your bloodstream and becomes usable by the body

Turkovic et al., 2004)