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Each trial involved placing the animal on a rotarod revolving at 5 rpm/min, then accelerating at 0.6 rpm/min

Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

Moderate responders form the largest group, experiencing significant improvement between weeks 3-6

BPC-157 consistently accelerates re-epithelialization across wound models, which may contribute to improved final appearance of healed tissue