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These enzymes typically have two domains: one that binds to glutathione and one that interacts with the target molecule
Glutathione in these fractions were alkalized and derivatized with 6-aminoquinolyl-N hydroxy succinimidyl carbamate as described previously
2A, compared with VRK2-KO cells downregulating the paralog genes involved in the GSH metabolic pathway, VRK2-KO cells with GCLC, GSS, and especially SLC7A11 knockdown exhibited increased ROS level, decreased GSH level and cellular viability
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