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Both the Tet-inducible vector pRMC2 (Addgene, plasmid 68940) 70 and the mKate insert were digested with KpnI-HF and EcoRI-HF (New England Biolabs), gel purified using a QIAquick PCR purification kit (Qiagen), and ligated using T4 DNA ligase (New England Biolabs) at 16 C for 16 h

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While traditional medicine relies on specific regulatory types to handle treatment based on symptoms and the patient's general profile, it is also crucial to recognize cross-cultural factors in preventive and therapeutic approaches, particularly understanding shared and distinct pathological, physiological, and psychological features (44)