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In the available data, 5-Amino-1MQ has generally been reported as well-tolerated but generally well-tolerated in limited data is not the same as proven safe for everyone. Human safety data is still limited, long-term effects aren't fully characterized, and that uncertainty is itself a reason for medical oversight
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Cytokine levels were measured using enzyme-linked immunosorbent (ELISA) assay kits: Human 1L-10 ELISA Ready-SET-Go from Affymetrix (Cat# 88-7106) and Human TNF- Uncoated ELISA (Cat # 88-7346), Human IFN- Uncoated ELISA (Cat # 88-7316), and Human IL-6 Uncoated ELISA (Cat # 88-7066) from Invitrogen to quantity detection of cytokines
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