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Neutrophil isolation and ROS detection Neutrophils from SF were harvested by centrifugation at 4000 rpm for 10 min at 4 C [15]
Injection of GLP-1 directly into the central nucleus of the amygdala (CeA) of rats results in CTA (Kinzig et al., 2002) whereas injection into the PVN reduces food intake without eliciting CTA (McMahon and Wellman, 1998)
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