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Ratiometric image analysis was conducted using a Python script (available from The background was subtracted for each channel and the ROIs (=transfected cells) were determined by thresholding or with the Cellpose 40 algorithm in the channel of the FP
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However, this approach often fails to address the underlying reasons why the Candida overgrowth occurred in the first place
UMFA can accumulate when folic acid intake exceeds the bodys capacity to convert it into biologically active folate, particularly in individuals with reduced activity of the dihydrofolate reductase (DHFR) enzyme, which catalyses the first step in folic acid metabolism
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