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The PCR reactions were prepared for three primer pairs in which the internal transcribed spacer 1 (ITS), 5.8S, and ITS2 regions (primers: ITS1 and ITS4), the partial sequence of the gene tef1- (primers: EF-1F and EF-1R), and the partial sequence of the rpb2 gene (primers: fRPB2-5F and fRPB2-7cR) using the EasyTaq kit (Sinapse Biotecnologia) using the manufacturers recommendations
First, the label explicitly lists vitamin B12 (cyanocobalamin or methylcobalamin) as an ingredient
doi: 10.3389/fcimb.2023.1238005 86
Diabetologia (2017) 60(9):180112
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