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It has been reported that cystine starvation alone was sufficient to induce ferroptosis in mouse embryonic fibroblasts, which was associated with an outburst of ROS 31

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doi: 10.1152/ajpgi.00219.2004 47 GongL.GoswamiS.GiacominiK

Using the cDNA Reverse Transcription Kit with High-Capacity (Applied Biosystems, United States), reverse transcription of total RNA to cDNA was performed