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LCMS detection of ZZ1 Following treatment of cells with 5 M of ZZ1 for 5 h, cells were washed two times with PBS and lysed in 100 l of a 2:1:1 mixture of acetonitrile, methanol and water

Total protein was quantified via the Lowry method

Is MIC+B12 FDA approved

IIS regulates the nuclear distribution of FoxO proteins which are increasingly considered to represent unique cellular targets directed against human cancer in light of their pro-apoptotic effects and their ability to lead to cell cycle arrest [5, 14]