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3 pooled samples were taken from each experiment

Dysbiosis of the microbiome forms a new internal environment, under which it may affect the development of GERD from the perspectives of molecular mechanisms: microbial activation of Toll-like receptors, microbial stimulation of cyclooxygenase-2 expression, microbial stimulation of inducible nitrous oxide synthase, and activation of the NLRP3 inflammatory vesicle

Statistical significance between pairs of groups was assessed using an unpaired two-sided t-test, one-way ANOVA followed by the Dunnett multiple comparison test, or two-way ANOVA with the Tukey multiple comparison test, as indicated

J Clin Endocrinol Metab (2015) 100(2):34262