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Although single cell RNA sequencing analysis are required to unequivocally demonstrate concomitant decreased levels of E2F1 and GLP1R within the same cell, we can speculate that the long term loss of efficacy of Glp1r agonist treatment to restore glucose homeostasis in T2D patients may be related, at least partially, to impaired E2F1-dependent modulation of GLP1R expression
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