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The discrepancy is too large to be dismissed as a chance finding, suggest the researchers, but could be the result of publication biassome studies might have been prioritized for publication because of the positive nature of their results whereas the unpublished datasets used in this study would not have been affected by any failure to publish null results
To separate and quantify the conjugates GS-TNB and CYS-TNB, a gradient elution method was implemented using eluent A, 7/10/83 (v/v/v) methanol/100 mM K-phosphate buffer, pH 6/water and eluent B, 30/10/60 (v/v/v) methanol/100 mM K-phosphate buffer, pH 6/water
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