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If you notice blood in the syringe before you have injected, remove the needle, dispose of it, and start again at a new site
Error margins are higher for subgroups within the overall sample
However, it is important to note that erythrocytes generate ROS primarily as a result of (i) hemoglobin oxidation, which can be considered as an iron-mediated Fenton reaction, (ii) the activity of NAPDH oxidase and (iii) xanthine oxidoreductase, while in nucleated cells ROS are mainly generated in the mitochondrial electron transport chain
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