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2018) Cell pellets (from 1L insect cell culture) were thawed and suspended in 20mM HEPES pH 7.4, 50mM NaCl, 5mM CaCl 2 , 2mM MgCl 2 supplemented with cOmplete Protease Inhibitor Cocktail tablets (Sigma Aldrich) and benzonase (Merk Millipore)
Mice were placed in the center of the open field with lights on and then allowed to explore for 5 minutes
The substantial weight reduction observed with tirzepatide monotherapy (15-21% body weight in clinical trials) suggests that any perceived benefits likely reflect tirzepatide's pharmacological effects rather than synergistic action
doi: 10.1016/j.autrev.2020.102741 217 AliAShahSAZamanNUddinMNKhanWAliAet al
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