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After 8 weeks, the efficiency of reconstitution was assessed by flow cytometry analysis of blood using CD45.1 or CD45.2 antibodies as described previously (20)
The pellet was resuspended in 200 l of 8 M urea with 10 mM Tris (pH 8), incubated at 60 C for 30 min with shaking and centrifuged for 15 min at 18,000 g for protein quantification of the supernatant with the DC Protein Assay (Bio-Rad Laboratories)
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