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Immunoblotting Proteins were extracted from cultured SCD erythroblasts or spleen Ter119+ erythroblasts with modified RIPA buffer (150 mM NaCl, 50 mM Tris, pH 7.5, 1% NP40, 0.25% Na-deoxycholate) plus protease inhibitor cocktail (Sigma) and stored at 80 C up to processing
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Live cell imaging results showed that some cells exhibited a state of non-division or nonextension under Gln deficiency (Gln) conditions, whereas cells grew plum and well under Gln sufficiency (Gln+) conditions (Fig
During obesity-induced adipose tissue remodeling, abnormal secretion of proinflammatory factors and adipokines promotes tumor cell proliferation, invasion, and metastasis, accelerating tumor progression
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