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For cell stimulation, cells were pretreated with various concentrations of GHK-Cu of 1, 5, or 10 M for 18 hours, followed by stimulation with 100 ng/ml LPS for specific time periods

In the early years of GLP-1 RA use it was suggested, an increased risk of pancreatitis and pancreatic tumors related to the use of these anti-diabetics.26 However, large clinical trials and other subsequent studies did not confirm this finding accordingly, the US Food and Drug Administration (FDA) and the European Medicines Agency (EMA) concluded that a causal relationship between these drugs and the development of pancreatitis or pancreatic cancer could not be established.2731 Our study has several limitations