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Hereditary intrinsic factor deficiency is caused by homozygous or compound heterozygous mutation of the CBLIF (cobalamin binding intrinsic factor) gene on chromosome 11q12.1 (168

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The complex was purified using Ni-NTA affinity chromatography followed by ion-exchange chromatography (IEX) using a Capto HisRes Q anion-exchange column and then size-exclusion chromatography (SEC) using a Superdex200 column

This led us to a debate about how, when you know a bit more about B12 deficiency and Pernicious Anaemia than your GP surgery, it comes as a bit of a surprise when bureaucracy outweighs common sense