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5c and d)
An additional 500 L 1:1 methanol / ddH 2 O was added and the sample was centrifuged at 14,000 g for 10 min at 4C to remove beads and solid debris
Never check reconstituted peptides in airline luggage where temperature is not controlled
From the ASV table (Supplementary Table S1), sums of ASVs within each respective phylum or genus for each sample were calculated to establish the abundance of each phylum or genus per sample
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