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To isolate fractionated adipocytes, fresh gWAT was minced and digested for 1 h in PBS containing 10 mM CaCl 2 , 2.4 U ml 1 Dispase II (Roche) and 1.5 U ml 1 collagenase D (Roche)
This tiered approach would provide physicians with a range of options matched to patient needs
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Daily intraperitoneal injections of GGsTop, which has previously been shown not to impair tumour growth 47 , did not abolish kidney GGT activity (Extended Data Fig
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